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1.
Biotech Histochem ; 92(6): 445-449, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28862488

RESUMO

We investigated carbohydrate residues on the epithelial surface, in the epithelial cells and in gland cells of the tongue of the mole rat using histochemical methods. We used horseradish peroxidase-conjugated lectins from Helix pomatia (HPA), Arachishypogaea (PNA), Ulexeuropaeus (UEA I), Canavaliaensiformis (Con A). The most intense reactivity was observed in the keratin layer with HPA, UEA I and Con A, and in the epithelial cells with UEA I and Con A. In the glands, we found strong reactivity in serous cells with HPA and Con A, and in mucous cells with HPA and UEA I. PNA did not bind to epithelial or gland cells. Consequently, GlcNAc, fucose and α-D-mannose terminal glycoconjugates are distributed widely; GalNAc terminal glycoconjugates appeared in small amounts.


Assuntos
Lectinas/metabolismo , Ratos-Toupeira , Língua/química , Animais , Glicoconjugados/química , Glicoconjugados/metabolismo , Histocitoquímica
3.
Int J Pediatr Otorhinolaryngol ; 58(1): 69-73, 2001 Apr 06.
Artigo em Inglês | MEDLINE | ID: mdl-11249983

RESUMO

OBJECTIVE: To investigate bacteremia in patients who underwent tonsillectomy with a dissection method due to chronic tonsillitis, which may lead to dramatic results in patients that have a cardiovascular infection risk. In this study, it was explored whether the tonsil surface and the tonsil tissue interior microorganisms are different or not, and whether these microorganisms carry bacteremia development risk. METHODS: Forty patients were enrolled in the study in ENT Clinic, Firat University. A bacteriological research was performed on patients with no treatment with antibiotics for a month before the operation. Tonsil surface and deep tissue cultures were performed, and venous blood samples were taken for cultures before and after the operation. RESULTS: Surface and deep tissue cultures of tonsils of the cases represented a different result in 27.5% of the patients. No reproduction was observed regarding the blood cultures taken before tonsillectomy. Bacteremia was found in blood samples of ten patients (25%) taken immediately after tonsillectomy. CONCLUSION: Hence, it has been concluded that different microorganisms may be present among the tonsil surface and tonsil deep tissue cultures and that bacteremia may develop after tonsillectomy. Therefore, to prevent the possible dramatic outcomes after tonsillectomy the required measures during the preoperative period should be taken into consideration, especially in patients that have chronic tonsillitis with cardiovascular disease.


Assuntos
Bacteriemia/diagnóstico , Infecções por Bactérias Gram-Negativas/diagnóstico , Infecções por Bactérias Gram-Positivas/diagnóstico , Complicações Intraoperatórias/microbiologia , Tonsilectomia/efeitos adversos , Tonsilite/microbiologia , Tonsilite/cirurgia , Adolescente , Adulto , Antibioticoprofilaxia , Bacteriemia/etiologia , Sangue/microbiologia , Criança , Pré-Escolar , Doença Crônica , Feminino , Infecções por Bactérias Gram-Negativas/epidemiologia , Infecções por Bactérias Gram-Positivas/epidemiologia , Humanos , Incidência , Masculino , Cuidados Pré-Operatórios/métodos , Medição de Risco , Tonsilectomia/métodos , Tonsilite/diagnóstico , Resultado do Tratamento
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